Palabras clave
Reactividad cruzada
Anticuerpos IgE
Ácaros
Mohos
Bioinformática
Cómo citar
Resumen
Objetivo: Examinar la posible reactividad cruzada entre A. fumigatus y los ácaros, ambas importantes fuentes de alérgenos en regiones tropicales, mediante un enfoque bioinformático.
Métodos: Se recuperaron las secuencias de aminoácidos de alérgenos de Aspergillus fumigatus registradas en la base de datos del alergoma y se utilizaron como entrada para realizar un análisis PSI-BLAST contra el proteoma de Dermatophagoides pteronyssinus. Se seleccionaron para su posterior análisis los resultados con similitudes y valores de cobertura de consulta superiores al 25 y al 80%, respectivamente. La predicción de epítopos de células B se realizó mediante la herramienta Ellipro. Solo se identificaron los epítopos conservados entre ambas fuentes alergénicas y se visualizaron en superficies de modelos 3D obtenidas mediante modelado basado en homología.
Resultados: Doce alérgenos de Aspergillus fumigatus compartieron homología con proteínas descritas en D. pteronyssinus. Todos los modelos 3D obtenidos mostraron el plegamiento típico de la familia proteica a la que pertenecen. La proteína ribosomal L3, la chaperona molecular Mod-E/Hsp90, la proteína ribosomal ácida P2, la enolasa y la peptidil-propil cis-trans-isomerasa fueron los alérgenos con mayor puntuación de identidad (>60%). Se predijo que al menos cuatro epítopos lineales B eran compartidos entre los alérgenos y homólogos en D. pteronyssinus
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